Hi Sean, Thank you for your response, I have gotten the feeling from reading that this was the case. I have used limma previously to compare samples as you have mentioned however I was planning on creating a scoring type matrix that would create a score for multiple factors and I was hoping to convert the log2 gene expression value to a absolute value, as an example; up-regulation (1), no change (0) and down Regulation (-1) This could then be taken into account but I assume that this would need a scale in order to determine the genes/probes that fall into each of these categories?
Thanks again for your help, Jonathan
frma data is log2 transformed. Are you using the InSilico DB R package (http://www.bioconductor.org/packages/2.12/bioc/html/inSilicoDb.html) or the web interface (https://insilicodb.org)?