If you need help, contact me at am@bioidea.com.pl
Best, Agata
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Quick question, probably already asked (sorry in this case).
How can i set "Max target sequences" in NCBI BLASTn equal to 1? The minimum value I can set from the website is 10...
Thank you all for the help and for the attention.
You can do it only with local Blast - https://blast.ncbi.nlm.nih.gov/Blast.cgi?CMD=Web&PAGE_TYPE=BlastDocs&DOC_TYPE=Download
Best, Agata
If you need help, contact me at am@bioidea.com.pl
Best, Agata
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word of caution: do not use the max-target parameter without reading up on it. It may (/will) not always work as you expect it to be. For more info google around for it. Here (https://blastedbio.blogspot.com/2015/12/blast-max-target-sequences-bug.html) is a good start.
Perhaps what you really want is to set "-num_alignments" to 1 ?