different e-value from linux standalone VS blast website
Hi everybody. I am using blast standalone 2.2.28+ in westgrid with linux for comparing two sequences (one short) and I do my best to use the same settings as blast website so:
blastn -query Spacers.HTBN.txt -db human.gut.fasta -gapopen 2 -gapextend 1 -penalty -3 -reward 1 -dust no -evalue 10 -word_size 10
but the results I get from the website VS linux have different e-values?! what can be the reason? I do really appreciate your help.
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Ram
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masoud.namini •
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masoud.namini •
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The Expectation value depends on the database searched. Perhaps you're searching different databases.
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karl.stamm
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Can you post your results? Did you tell the web version to use BLASTN or megablast?