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fastqc report and quality check

I am conducting an RNA seq analysis pipeline for which I have to conduct fastqc report check. I am curious to know what is the exact significance of the parameter of the term per tile sequence quality. If this parameter seems problematic and other quality checks are okay can we still proceed with the fastq file?

rna-seq
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updated by Ram Ram • written by aranyak111 • aranyak111

You can't directly link files from your local desktop. Please upload them to imgbb dot com following directions in post above.

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written by GenoMax GenoMax

Please let me know if you can see the uploaded image and subsequently give your valuable opinion.

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written by aranyak111 • aranyak111

I changed that to a comment, because it is not an answer, and we can not see the image you uploaded, refer to GenoMax comment.

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written by Medhat Medhat

1 answer

It depends how bad it looks like, a bubble or areas with errors are ignored in the base calling, details: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/3%20Analysis%20Modules/12%20Per%20Tile%20Sequence%20Quality.html

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written by JC JC

Not the plot you wanted to show us.

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written by GenoMax GenoMax

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