I am having a difficult time finding information about a minimum threshold for number of individuals to use in wgs pop gen analyses. Are there software or formulas/theory available to determine what number total number of haplotypes/individuals you need to get theta estimates, make a linkage map, run mk tests etc based on inputs such as genome size, read depth per individual, sequencing platform? Obviously there can financial constraints and considerations to make for non-model organisms when choosing how many individuals to sequence. Any advice would be appreciated.