how treat FLAG 16 in ribosome footprint
hello. first, sorry for that I'm not good at this field. I aligned reads to reference, and now I'm finding each read's start location from start codon to draw ribosome footprint figure. My question is
how to treat FLAG 16. I understand that they aligned to reverse strand, so they expressed in reverse complement forms in sam files. so if i have
read1thatPOS:25andFLAG:16, is it correct to pointread1in 25 location from start codon in ribosome footpring? Just treat like other flag0 reads?why we have reverse strand sequence in reads?
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