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Cell type annotation in Sc-RNA Seq data

Greetings,

I have single-cell sequencing data from three different Patients(hepatocellular cancer). I'm trying to annotate the cell types for each cluster using SingleR and Celldex libraries.

I have Gene Ensemble Ids in Seurat object or Sce object. I don't know how to modify hpca.se to have ensemble ID instead of gene names.

Any recommendation or other tools for cell-type annotation would be appreciated.

singler celldex cluster rna-seq r

1 answer

Try setting ensembl=TRUE when loading the hpca.se data set (see the celldex vignette). This way the gene names should be returned in ENSEMBL format.

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