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confirm assembly in pan-genome analysis

hello everyone, after assembled genome for design pan-genome in which one of the stages we can confirm famous genes sequence after assembled each genome,?or after assembled all of the genomes? everyone has experience in this matter(design pan-genome), please take comments for me.

tnx.

best wish

assembly genome sequencing

Sorry, I think you need to rewrite your question to make it clear. MAybe bullet points might help? Bacteria? Human ? Data available ? Goal ?

genome=buffalo genome,number of genome=14 whole genome, goal:confirm assembly

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