Entering edit mode
3.5 years ago
ecg1g15
▴
30
I have three groups of environmental RNA- Seq data (5-6 replicates each) and would like to see the differential expressed genes between them (ie: genes that are DE in L1, in L2 and in L3). I have seen many statistical methods use a control to compare to, but I don't have control since I am comparing environmental data; and tests between only two groups, where I have 3. What would be your best recommendation. I have 3 observations (5-6 replicates each) and about 5000 genes.
Compare L1 vs L2, L1 vs L3 and L2 vs L3, that are the pairwise comparisons you can make. All of the tagged tools support that.