Hello everyone :)
I need help from Single-Cell multi modal enthusiasts! We have performed 10x 5' seq -- TCR seq, CITE-seq and gene expression of PBMCs.
To get a closer look a CD4 enriched fraction of the PBMCs was also taken and same multi modal seq was done.
And each patient has two time points.
So now, I have 4 hashtags (oligonucleotides) for the 2 diff time points and for two levels (whole PBMCs and enriched fraction) apart from all the hashtags for the CITE-seq .
Essentially what I have now is only one resultant matrix file for each person at each modality (or 2 fastq (r1 and r2) for gene exp and so on).
How do I get back the information on which time point and which level of enrichment things belong to?
0 answers
No answers yet.
Log in to answer this question.
-
Longitudinal analysis of subpopulations: which approach is better?
written by Lluís R.
-
Empty VCF file from Mutect2 v. GATK 4.1.6.0 in tumor only mode
written by pm2012
-
Error running summarizeToGene() - RNA Seq Bioconductor pipeline
written by TonyCN •
-
Masurca genome kunitg not finishing
written by olivier_armant •
-
Do I have adapters in my reads?
written by ntsopoul •
-
Interpreting bbduk histogram headers and contents
written by gnmcsbnfrmtcsclb •
-
RNA-Seq time series analysis using a DESeq2 spline approach yields far too many significant genes
written by stu111538
-
Splitting RNA-Seq data set on two (three) flow cells
written by scheitelt •
-
Help with multi-factor Deseq analysis
written by MAPK
-
Speed up Tophat2 running
written by yuabrahamliu •
Did you ever figure out how to quantify the HTOs?