Solved with umi-tools, Thnx!
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a.almeida •
How to trim UMI and add it to read name line?
Hello there,
I am getting started in 5cap-seq analysis (mapping of TSS sites)...
After demultiplexing I have to trim the first 8 nucleotides (UMI) of the 5' end and add them to the read name line. This will be useful for deduplication in a later step.
Can somebody help me how to do this? I think I could use Cutadapt --cut to trim away the 8 nt but then how can I preserve these in the read name line?
Thank you!
p.s.: I am only familiar with unix and R
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