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Is GSEA analysis suitable for the research without genome?

As the title description,

I am going to do GSEA (Gene Set Enrichment Analysis) analysis for evaluate the RNA-datasets and classify gene annotation information.

However, my experiment specie was not sequenced for the genome. I want to ask if GSEA (Gene Set Enrichment Analysis) analysis suitable for the research without reference genome.

But I now have the BLAST nucletide annoatation and know every transcripts probably represnting. Is this useful?

rna-seq gsea gene set enrichment analysis

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