Online alignment for large fasta files
Hi
I have a fasta genome file that contain 4000 genomic sequences (~ 30Mb size) in it. I would like to align this 4000 sequence to a reference genome. I was trying to use SINA (reference base multiple sequence alignment) in galaxy (https://usegalaxy.org/) however I have got memory error. Is there any online sources that I perhaps can use for this purpose?
Thanks,
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If you only need to align the sequences to a reference, I'd suggest
blastnhttps://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome
You may also be able to use mummer: http://mummer.sourceforge.net/
If you have sequencing reads, you can use
bowtie2.