I want to identifying consensus peaks from several biological replicates of a ChIP-seq experiment using IDR (Irreproducibility Discovery Rate) and for that I need to …
In several manuals ([example][1]) on ChIP-seq analysis they pre-select, for instance +1000bp and -1000bp from the TSS as the "promoter-bound" regions: ```r peakAnno_bcl11b <- ChIPseeker::annotatePeak(peak …
Like the title says. I've seen this mentioned as a problem before (for example, [this page][1] states "*blacklisted (repeat) regions with ultra high signal will …
In the process of analyzing ChIP-Seq replicates with DiffBind (drosophila data, 3 replicates for each sample, choosing DESeq2 as the analysis method), peaks with very …
Do discordant alignments exist in those tutorials? Usually, ChIP-seq experiments are single-read (not paired-end) so that is not an option.
These tutorials are indeed single-read. Silly me.