This is a test version of Biostars. For the public version, visit https://www.biostars.org.
Should discodant alignments be retained in ChIP-Seq experiments?

In some online tutorials for ChIP-Seq (1,2) after aligning, non-unique reads are filtered out. However, discordant alignments are not.

Is it standard practice, and why, if so?

chip-seq

Do discordant alignments exist in those tutorials? Usually, ChIP-seq experiments are single-read (not paired-end) so that is not an option.

These tutorials are indeed single-read. Silly me.

0 answers

No answers yet.

Log in to answer this question.