Which reference genome file is correct?
I am implementing a bulk RNAseq pipeline to convert fastq reads into a count matrix. I would like to download the latest human reference genome to align the NGS RNAseq reads using hisat2. I've found several ways to download what I think is the reference file (size: ~40MB), but it seems to be much smaller than I remember reference files being (>1GB). Is "Homo_sapiens.GRCh38.101.gtf.gz" the correct reference file or can someone sent me a link for the correct human reference genome?
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GenoMax
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theodore.killian •
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theodore.killian •
1 answer
That is just the reference annotation in GTF format. You will need that as well. You can download corresponding primary assembly sequence fasta file here.
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