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How to do linkage disequilibrium analyses?

Hello, I have the alignment of the different alleles (~40 alleles) of a single gene in a Fasta formatted file. Is it possible to do a linkage disequilibrium analysis? And make one of those trivial triangular LD plots? Please let me know if it is possible to do with any specific software/tool. Thank you.

alignment sequencing gene r snp

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