Dear all,

I'm using BBDuk from bbmap package (last version) for the trimming of RNA-seq reads. I used the below code:

bbduk.sh in=file_1.fastq.gz  out=trimmed_1.fastq.gz  ref=/opt/BBMap/resources/adapters.fa ktrim=r k=23 mink=11 hdist=1  qtrim=rl trimq=20

But it gave the error:

java -Djava.library.path=/opt/BBMap/sh/jni/ -ea -Xmx11756m -Xms11756m -cp /opt/BBMap/sh/current/ jgi.BBDukF in=file_1.fastq.gz out=trimmed_1.fastq.gz ref=/opt/BBMap/resources/adapters.fa ktrim=r k=23 mink=11 hdist=1 qtrim=rl trimq=20
Error: Could not find or load main class jgi.BBDukF

From the net, I tried the below solution code:

java -ea -Xmx5g -Xms5g -cp /opt/BBMap/current/jgi.BBDukF in=file_1.fastq.gz  out=trimmed_1.fastq.gz  ref=/opt/BBMap/resources/adapters.fa ktrim=r k=23 mink=11 hdist=1  qtrim=rl trimq=20
Error: Could not find or load main class in=file_1.fastq.gz

I'm totally confused. Could you please let me know how I can fix the error?

Thanks