How to find differential gene expressions
Hi, I am a mathematician and new to mRNA analysis. I am analyzing some mRNA seq data. My data set contains four-time points. in each time-point, I have 200 cells and 10000 genes. I need to find the differentially expressed genes between each time point. First of all, I would like to know, how and by which method I can find these genes? I mean statistically how it works? Second, I would like to convert this data set as annData in scanpy, but I do not know how should I do it? I would be thankful if you can give me a hand on it.
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Please read through the manuals of e.g. scanpy, Seurat or the Bioconductor single-cell workflow (OSCA) to get started. Biostars is great for specific questions but generally it is expected that you read available resources first to get a background.
Hello zf.math2013!
I will close this for now as there is no specific question, but feel free to comment if you want this reopened.