GSEA single cell
Hello, I am analyzing single cell data. I ran Seurat's FindMarkers function to find the differentially expressed genes between each diseased and healthy celltype. I wanted to do Gene set enrichment analysis using clusterProfiler package. How to rank genes before using it.
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I use
sign(log2Fold )* -log10(pvalue), if you use presto you may use auc to rank, see https://crazyhottommy.github.io/scRNA-seq-workshop-Fall-2019/scRNAseq_workshop_3.htmlHi. I am looking for paper or justification about using :
Do you have any ? Thanks