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protein database peptide identification

Hello all, I have a list of peptides from MS/MS analysis and I want to compare it with a known protein database to find novel peptides. Can somebody help me with some script or whatsoever? Thank you

sequence alignment

1 answer

There are two options:
- Map your peptides to a reference proteome
- Search a database of previously reported peptides (e.g. PRIDE, maybe try its peptidome)

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