This is a test version of Biostars. For the public version, visit https://www.biostars.org.
BBMap Scaffstats Understanding of Output

Hello,

I would like to understand the meaning behind BBMap output scaffstats:

%unambiguousReads
unambiguousMB
%ambiguousReads ambiguousMB unambiguousReads
ambiguousReads
assignedReads
assignedBases

assembly sequencing

1 answer

Unambiguous = Mapping to one reference sequence
Ambiguous = Mapping to more than one reference sequence
Read and Bases are just that.

Thank you for letting me know

For instance:

name %unambiguousReads unambiguousMB %ambiguousReads ambiguousMB unambiguousReads ambiguousReads assignedReads assignedBases

NZ_GG773477.1 Escherichia coli MS 116-1 Scfld976, whole genome shotgun sequence 4.93292 31.023300 0.00021 0.001350 206822 9 208715 31307250

This means that 4.9% of the input read aligned to the shotgun sequence? On the same token 0.001350 % is ambiguous, matching to more than one reference sequence. For the latter, how does bbmap determine that the read has mapped to more than one reference sequence?

Log in to answer this question.