mapping quality scRNA seq
Hey everyone,
I am working with single-cell RNA seq data and now I want to check the mapping quality. I've read about couple of tools I can use, like fastqc,RSeQC etc My question is which is better and how I choose if I need to filter out some reads?
Thanks in advance
Anna
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What program are you using for analyzing your scRNAseq data? e.g. if you have 10x data you may want to use the statistics produced by
cellranger.My plan is to use Seurat
Sure but
seuratis for secondary analysis. What are you using for alignment of the data?oh ok. Hisat2 with argument
-k 1