detecting UMIs barcodes
Hello,
I started to analyze single cell RNAseq data recently. I am super new in this field and I want through my question to clarify some things which confuse me. So my question is about the UMIs barcodes. How can I detect then/find them in the reads without knowing something about them i.e length/pattern etc.
My data are coming from Smartseq2
Thanks in advance Anna
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Do you know for sure that UMI's were added to your Smartseq2 samples (ref: http://seqanswers.com/forums/showthread.php?t=75946 )?
There are no UMI's shown here either: https://www.illumina.com/science/sequencing-method-explorer/kits-and-arrays/smart-seq2.html