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How to check the conservancy of DNA sequence.

I have a small DNA segment and my dataset has almost 100 long DNA sequences. I want to check whether this DNA segment is conserved among all DNA sequences in my dataset. The conservancy result should be in a percentage (like it is 90% or 100% conserved). IEDB Epitope Conservancy Analysis tool does the exact same thing that I want, but it is for protein sequences.

Is there any such tool for DNA sequences?

dna conservancy

All DNA residues - A, G, C and T - are legitimate protein residues as well. If percent identity is all you care about, enter your nucleotide sequences into that form and pretend they are proteins. It should work.

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