Thank you very much for your reply , so i need to create file ans specefy all this things right , and other thing , i have just fastq files reads , so i shouldn't put this lines
fasta file for read 1
f1=/path/LIBNAMEA/fasta_read_1.fa
fastq file for read 2 always follows fastq file for read 1
f2=/path/LIBNAMEA/fasta_read_2.fa
# fastq file for single reads
q=/path/**LIBNAMEA**/fastq_read_single.fq
# fasta file for single reads
f=/path/**LIBNAMEA**/fasta_read_single.fa
# a single fasta file for paired reads
p=/path/**LIBNAMEA**/pairs_in_one_file.fa
and this lines
q=/path/**LIBNAMEB**/fastq_read_single.fq
f=/path/**LIBNAMEB**/fasta_read_single.fa