How to covert RKPM into absolute quantification?
Hello, is it possible to convert RPKM values into absolute values? That is to convert a qualitative (relative) measurement into a quantitative (absolute) measurement? If I have an RPKM of, let's say, 375 for a gene of interest (or micro-organism) and the sample was 1 mL of something, can I calculate the number of initial copies of the gene?
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marongiu.luigi
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This is not possible with regular RNA-seq data.
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Devon Ryan
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If you don't have a spike-in of known concentration is impossible. See answers to a similar question here