Entering edit mode
4.2 years ago
Bogdan
★
1.4k
Dear all,
i would like to ask for a suggestion about the design of guideRNA for CRISPRko, CRISPRi, or CRISPRa experiments.
we are using CHOPCHOP https://chopchop.cbu.uib.no/ to design guideRNAs for CRISPRi , CRISPRko, CRISPRa experiments that target ENHANCER regions ;
would the guideRNAs used for CRISPRko work for CRISPRi too (given the fact that CRISPRi uses dCas9 enzyme) ?
CHOPCHOP predicted for us the same guideRNA for either CRISPRko, CRISPRi, CRISPRa ... thanks a lot !
-- bogdan