A mirtron on my small RNA-seq data
Hello,
As I have finished the differential expression analysis of my small RNA-seq data, I found on mirtron on my DE miRNAs. And it is only specific to the Sus scrofa species and not annotated in humans. Is this normal to find a mirtron on my small RNA-seq data or maybe is this just a false positive? I've used DeSeq2 to analyze my data.
Thank you.
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Why not? Obviously you'll have to verify its expression in the lab
Thank you for the replies and very funny with the megatrons haha.
One more question, if this is not annotated in other species, do you have any strategies on how can I predict its target genes as well? Since on miRBase, it does not have much mention on papers - actually just one.
For those who are not familiar with mirtrons.
Not to be confused with megatrons
I actually thought it refers to all miRNAs that reside in introns, including the ones transcribed with their own promoter and going through the traditional DROSHA-dependent splicing. How can you tell it's DROSHA-independent from sRNA-seq?