FastQC K-mer Content Peaks at 3' and 5' end
Hi, I'm fairly new at FastQC. I removed bad quality transcripts and illumina adapters with sickle and scythe. However, even after trimming I still have peaks of K-mer content at the 5' and 3' end.
Any ideas as to what these peaks can be attributed to other than just "contamination"?
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lekenyon •
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I am wondering what exactly you removed. Can you post the pre-trimming image so we can see what the original data looked like?
Have you checked these informative blog posts from authors of FastQC. Link1 and Link2.