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galaxy prinseq trimmming sequence

Hi friends, I run prinseq in galaxy with same parameters for my file with 6 sequences twice: why results is different?

this is the main file:

test-trim

this is the result with sliding window end trimming (both ends) = 1 base window, 1 base step size:

test-trimgood-1

this is when sliding window end trimming (both ends) = 10 base window, 3 base step size:

test-trimgood-1good-2

sequence trimming prinseq
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written by Rob • Rob

I don't understand your question, first you say that you use the same parameters, but then you say that you use different parameters of windows and step size.

Also, it is not very clear what you expect and why you are concerned about your results exactly. It is not easy to process through big chunck of text pasted as image, so if you could be a little bit more specific, it would help.

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written by Carlo Yague Carlo Yague

Thanks Actually I just want to learn about the different trimming and how they change the sequences and what is their capability. Overall I have same settings just the sliding window ans step size I change to see what affect they have

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written by Rob • Rob

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