Hi All

I need help regarding how to format the sample sheet file (required for demultiplexing using bcl2fastq) for single index data. Usually I have dual index data and I keep below column names in the sample sheet.

• Sample_ID

• Sample_Name

• Sample_Plate

• Sample_Well

• I7_Index_ID

• index

• I5_Index_ID

• index2

• Sample_Project

• Description

Now, this time I have single index data and I am wondering whether I need to -

[1] Keep the i5_index_ID & index2 columns but keep them blank

OR

[2] Keep the i5_index_ID & index2 columns and add some text (NA)

OR

[3] Delete i5_index_ID & index2 columns

Please help.