Entering edit mode
4.4 years ago
gracie
▴
20
Hi all,
I am dealing with a Z normalized expression dataset and I want to perform differential expression analysis with two factor . I tried limma with two factor design (and also single) but couldnt find any gene altered significantly.
I am not sure is it because I am violating any assumptions of limma. Is there any other way for such analysis or any suggestions for the normalization of custom made microarray?
Thanks in advance
Why not simply feeding the raw data into the common microarray workflows?