RNAseq recommendations / terms read pairs vs. reads for paired end experiments
1 answer
The number of reads sequenced doesn't change between single vs paired end sequencing. In paired end you sequence both ends. So if the recommended is 10-25m, that can be single or paired end. Of course PE is the better option, and strandedness is even better, so is larger read size.
The recommendation changes whether you are doing DE, looking for novel transcripts etc etc
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