miRWalk and Cytoscape to show microRNA-mRNA interactions
Hi All,
I have about 5 miRNAs and each of them I have some confirmed mRNA target, I want to use the following figure to show the network among these miRNA and mRNA. Is there any protocol?
Thanks.
mirwalk
cytoscape
microrna-mrna
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Shicheng Guo
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Dear @Shicheng Guo
Do you already have mapped the targets?
If so, create a txt file with the miRNA-target and import into cytoscape.
After that you will need to read a little in cytoscape's manual and use your creativity.
You can also see a brief explanation of how to deal with cytoscape here:
A: Cytoscape network of upregulate and downregulate transcription factors: how to d
If you have not mapped the targets....
You can get some insights here:
C: MicroRNA-target interactions databases
Thanks Leite, Your suggestion is really helpful!!