alignment tool for big data
Length of my sequence is 7000000bp and I want to align with other sequences which tools are good for this
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Ram
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gbahramali •
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gbahramali •
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I want to drow phylogenetics after multiple sequence alignment
Please use
ADD COMMENT/ADD REPLYwhen responding to existing posts to keep threads logically organized.SUBMIT ANSWERis for new answers to original question.same question: how many genomes from BLAST hit should be considered for phylogeny tree analysis?
(Open the link, the title does not describe the question very well)
The 'answer' to this is you don't align sequences of that size, certainly not for multiple sequence alignment and phylogenetics.
You need to use an indirect approach such as aligning orthologues, or computing a
mashtree.Have a look at minimap2.
Not for MSA...
You are right. That was not obvious for me from the initial question.