Hi,
I would like to use Harmony to remove batch effects from my 10x Genomics scRNA-seq data combined from three donors. However, I am wondering whether Harmony requires the same cell populations to be present in all samples, or how it deals with a population that is e.g. unique to one donor?
Many thanks,
Lucy
2 answers
No. They illustrate that a point with the main figure on their tutorial page:

Harmony uses fuzzy clustering to assign each cell to multiple clusters, while a penalty term ensures that the diversity of datasets within each cluster is maximized.
Thanks, this shows cell types that are present in two out of the three datasets. Does this work equally well if one of your populations is only present in a single dataset?
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looks like it requires same population.