scRNAseq: How to select your reference dataset for cell type identification
HI guys, Sorry that's gonna be a pretty naive question, I am trying to the cell types coming from my sample (Mice Hippocampus) and am kind of lost as to what reference dataset I should use. I've created my tSNE plot using Seurat but I don't know how to proceed to identify my clusters.
Cheers and thanks.
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You might want to check SingleR (https://github.com/dviraran/SingleR) and especially its new dev version here: https://github.com/LTLA/SingleR