Thank you RaghuM :) I'll dig into FASTX-Toolkit more seriously!
Hi,
I am looking for a powerful and flexible tool to trim 454 sequences. I would like it to be able to remove the following:
- Tags
- 454 adaptors
- Low complexity regions
- Poly-A/T
- Low quality regions
The sequences can be either in .sff or .fasta/.qual format.
I'm keen on knowing what you guys would recommend and why :)
Cheers!
2 answers
Try NGS_Backbone: It can clean sanger, 454 and illumina sequences.
http://bioinf.comav.upv.es/ngs_backbone/cleaning.html#clean-reads
and also "FASTX-Toolkit"
For Illumina data. I use fastx toolkit to do some of the above analysis which you are looking.
For example
Quality filter at Q 12 and atleast 50% good bases and End trimming with quality filter at 12 and minimum length (30 bases) of the read to retain the read.
fastq_quality_filter -q 12 -p 50 -i Input_reads.txt | fastq_quality_trimmer\
-t 12 -l 30 -o filtered_reads.out.txt
my barcodes are 8-bases long
cat filtered_reads.out.txt | fastx_barcode_splitter.pl --bcfile mybarcodes.txt\
--bol --exact --prefix filtered_reads.out.txt.
fastx_trimmer -f 9 -i filtered_reads.out.txt.Tag1 \
>filtered_reads.out.txt.Tag1.txt
Please someone list me the list of 454 sequence quality trimming softwares. I tried fastx toolkit (fastq trimmer and fastq filter), clean_reads, PRINSEQ. Anyother softwares?
Hi @Ram. If you have a question to ask on the forum, please create a new question rather than post it as an answer within another question. Take the time to make a search on the forum in case such a question would already exist. Cheers
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