Hello,

My colleagues and I have human, whole blood RNA-Seq data from which we would like to estimate the proportions of various immune cell types within each sample and then see how they are changing between samples. We do not, however, have our own matched single cell RNA-Seq data set to help with the deconvolution. What methods would people recommend we consider? We're particularly interested in any methods that have already been validated to work well on whole blood samples.

Thanks!