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question about signal of IP / Input

I wonder why the log2(IP/Input) value of the distal region is not about 0. For example:enter image description here

The code used to generate the bamcompare file is:

bamCompare --scaleFactorsMethod readCount --minMappingQuality 1 --samFlagExclude 256 --operation log2 --outFileFormat bigwig --binSize 20 --pseudocount 5 -b1 Input.bw -o IP.bw

I think the possible reason is: There are too many reads in some unknown region in Input sample. Is this explanation reasonable? Or there are other reasons?

chip-seq
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written by ch8316f5eyu • ch8316f5eyu

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