DNA Sequencing of Single Genome Amplicons analysis
Hello all,
I have a few DNA sequencing reads (Illumina) for HIV amplicons that are supposed to derive from single genome amplification. I have picked the samples based on the band size when run on my gel and now I'd like to confirm that my sequences are indeed coming from a single genome and that they are the gene that are supposed to be. Which way do you think should be the best?
Thank you!
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
Best Way to Assemble DNA Sequencing Data
written by echolley •
-
full lenght gene amplification from RNA seq data
written by maryum •
-
targeted DNA sequencing
written by Pettorato •
-
Library Prep for NGS
written by Sujata •
-
Consensus protein from aavf
written by pennakiza
-
Multiple Displacement Amplification (MDA) for amplifying parasite genome.
written by anuragmishra1009 •
-
Band in Negative Control PCR, but no band in one sample with template added? Primer dimers?
written by Nicolai Skovbjerg Arildsen •
-
Discrepancy between agarose gel and bioanalyzer after sonication for ChIP
written by augelloma •
-
Just need to learn to do one thing: counting the reads that map to the human mitochondrial genome
written by viottimanuel •
-
Gel Electrophoresis of Mitochondrial Genome
written by boliyu-131 •
How many sequences/amplicons are there? One option is to align to a reference and call SNP.
I have 96 amplicons - I consider each one of them a different sample although they come from the same patient. I'm just a bit worried about SNP calling, as it's HIV Env and I am not sure how accurate would that be, considering the high variance in different quasi species? I'm sorry if I sound silly, I have just started working with DNA seq and HIV!