Hello everyone! I am using MaxEntScan for evaluate the effect of a SNV in splicing scores. However, I don't understand its output. I've read in Human Splicing Finder that if there is a variation of +/-30% between WT and Mutant the mutation creates a new splice site or breaks the splice site, respectively. Is it correct? I've not found this information in other references.
On the other hand, I'm not sure in the cases that the variant is located at the end of exon that It is transcribed in reverse strand. I consider that I should evaluate the score5ss for human 5' splice sites (3 exonic flanks and 6 intronic flanks around the 5'ss). I might be wrong. Could someone help me?
Thanks in advance.