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Can anyone suggest me "How to predict smallRNAs from p.aeruginosa whole genome data"?

Hi EveryOne,

I Have 5 P.aeruginosa Complete NGS whole genome data and I am trying predict smallRNAs from it. The raw data was assembled and annotated. Already I tried BSRD I got 80 smallRNAs but the database was updated very long back. So, now I trying manually, till now I have extracted intergenic regions from annotated gbk files followed by RHO-terminator seq filter. What i can do more to comeup with the best hits of smallRNAs. Thanks in advance.

genome next-gen sequencing sequence

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