Hello,
I m french student who's trainee in a neuroscience lab and I m stuck for months with a problem. I m explaining myself : we have eleven complete human genome, and I want to know if they are something different in those DNA like a virus who's integrated or some known mutation. For this, I did some research and I tried to blast those data in galaxy using the server of the lab. I also have two version of each genome : FastQ and FastA. Each FastQ genome weighs about 25GB and for the FastA it about 15GB so it really heavy to do something. I had an idea : just blast the entire genome of my patient with the reference human genome and look what's happen and if they are differences search what they are (viruses, mutations..) but then I had a problem : in galaxy I don't know how to get a reference human genome... I'm not at all a bioinformatician, actually I'm a medical student and I don't have the basics of tools for doing that, but I'm trying to do my best to resolve this problem and to progress in this domain with your help :)
I'm really sorry for my approximative english and I hope someone will could help me ! Thank you very much for your time !
Why do you do this all alone ?
I'm not doing that alone I have the help of the members of the research team ! But it my personal project to analyze those genome in order to learn some skills in bioinformatics
I see. So follow genomax's suggestion: forget galaxy and learn the unix command line.