Normalization with BBnorm
Hello All,
I am trying to perform a co-assembly from 2 samples that have been sequenced across two lanes of Hi-Seq, but I am not able to do so because of computational limitation problems. I have came across BBnorm where it is possible to perform digital normalization.
How do I determine the median coverage from my set of data, to set as the target coverage?
Will it be possible to leave out the minimum target coverage as well, as I have performed mapping and some of my contigs have a coverage of 1-2.
Thank You.
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
Forum: Recommendation required for solution of computation requirements in Genomics field
written by hafiz.talhamalik
-
WGCNA coexpression of deisased related module
written by mahmudaga •
-
lineage analysis on microbial samples (tuberculosis)
written by hafiz.talhamalik
-
Coverage for a specific gene in genome assembly
written by sarah.goldstein •
-
How much sequencing depth is required to detect a rare group of organism in environmental sample?
written by Ming •
-
Tools for viral metagenomics profiling and abundance estimation using BAM file
written by rice.researcher
-
How to measure NGS depth coverage bias
written by Anand Rao
-
Using rnaseq reference sample across batches
written by denalitastic •
-
Calculate percentage of bases covered less than nX in targeted sequencing experiment
written by Ram
-
Optimum on-target coverage for exome-sequencing
written by hellbio
