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average nucleotide identity (ani) of fungi

Hello everyone how can I use ANI (average nucleotide identity) for eukaryotic genomes (fungi)

alignment genome

What do you mean by "use"? Your question is too vague, please edit it and add more detail.

I am trying to calculate ANI between different species of fungi that have a chromrsome number different from one species to another, how can I do thank you

can I find software that allows me to calculate ANI between fusarium species

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I think you might be able to use GET_HOMOLOGUES to calculate ANI based on BLAST results. I'm not completely sure as I only calculated ANI for bacteria using this software. The program also prints an image and you can configure the layout of the heatmap. Here is the link if you wanna give it a read and investigate whether it fits your purpose: https://github.com/eead-csic-compbio/get_homologues (software), http://eead-csic-compbio.github.io/get_homologues/manual/ (manual).

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