Hi All,
Could you kindly tell me which is the difference between the two sequnces below?
http://www.ncbi.nlm.nih.gov/protein/9587714 http://www.ncbi.nlm.nih.gov/protein/7230482
They are both a cxc chemochin type 4 of the same organism: http://en.wikipedia.org/wiki/CXCR4
Why are the aminoacid in the two sequences different?
3 answers
They both align almost perfectly.
Score = 681 bits (1757), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 331/334 (99%), Positives = 333/334 (99%), Gaps = 0/334 (0%)
Query 9 EEMGSGDYDSIKEPCFREENANFNKIFLPTIYSIIFLTGIVGNGLVILVMGYQKKLRSMT 68
EEMGSGDYDSIKEPCFREENANFNKIFLPTIYSIIFLTGIVGNGLVILVMGYQ+KLRSMT
Sbjct 1 EEMGSGDYDSIKEPCFREENANFNKIFLPTIYSIIFLTGIVGNGLVILVMGYQEKLRSMT 60
Query 69 DKYRLHLSVADLLFVITLPFWAVDAVANWYFGNFLCKAVHVIYTVNLYSSVLILAFISLD 128
DKYRLHLSVADLLFVITLPFWAVDAVANWYFGNFLCKAVHVIYTVNLYSSVLILAFISLD
Sbjct 61 DKYRLHLSVADLLFVITLPFWAVDAVANWYFGNFLCKAVHVIYTVNLYSSVLILAFISLD 120
Query 129 RYLAIVHATNSQRPRKLLAEKVVYVGVWIPALLLTIPDFIFGNVSEADDRYICDRFYPND 188
RYLAIVHATNSQRPRKLLAEKVVYVGVWIPALLLTIPDFIF NVSEADDRYICDRFYPND
Sbjct 121 RYLAIVHATNSQRPRKLLAEKVVYVGVWIPALLLTIPDFIFANVSEADDRYICDRFYPND 180
Query 189 LWVVVFQFQHIMVGLILPGIVILSCYCIIISKLSHSKGHQKRKALKTTVILILAFFACWL 248
LWVVVFQFQHIMVGLILPGIV+LSCYCIIISKLSHSKGHQKRKALKTTVILILAFFACWL
Sbjct 181 LWVVVFQFQHIMVGLILPGIVMLSCYCIIISKLSHSKGHQKRKALKTTVILILAFFACWL 240
Query 249 PYYIGISIDSFILLEIIKQGCEFENTVHKWISITEALAFFHCCLNPILYAFLGAKFKTSA 308
PYYIGISIDSFILLEIIKQGCEFENTVHKWISITEALAFFHCCLNPILYAFLGAKFKTSA
Sbjct 241 PYYIGISIDSFILLEIIKQGCEFENTVHKWISITEALAFFHCCLNPILYAFLGAKFKTSA 300
Query 309 QHALTSVSRGSSLKILSKGKRGGHSSVSTESESS 342
QHALTSVSRGSSLKILSKGKRGGHSSVSTESESS
Sbjct 301 QHALTSVSRGSSLKILSKGKRGGHSSVSTESESS 334
The shift comes from the fact that those sequences were extracted from a partial RNA:
gi:7230482 is coded by a partial DNA : http://www.ncbi.nlm.nih.gov/nuccore/7230481
gi:9587714 is also coded by a partial cds : http://www.ncbi.nlm.nih.gov/nuccore/AF172229.1
These can be two variants of same gene: As the title says one is CXCR4 homologues of gibbon ape. Other possibility may be use of different start sites.
CLUSTAL 2.0.12 multiple sequence alignment
AAF89349.1 IYTSDNYTEEMGSGDYDSIKEPCFREENANFNKIFLPTIYSIIFLTGIVGNGLVILVMGY
AAF42991.1 --------EEMGSGDYDSIKEPCFREENANFNKIFLPTIYSIIFLTGIVGNGLVILVMGY
****************************************************
AAF89349.1 QKKLRSMTDKYRLHLSVADLLFVITLPFWAVDAVANWYFGNFLCKAVHVIYTVNLYSSVL
AAF42991.1 QEKLRSMTDKYRLHLSVADLLFVITLPFWAVDAVANWYFGNFLCKAVHVIYTVNLYSSVL
*:**********************************************************
AAF89349.1 ILAFISLDRYLAIVHATNSQRPRKLLAEKVVYVGVWIPALLLTIPDFIFGNVSEADDRYI
AAF42991.1 ILAFISLDRYLAIVHATNSQRPRKLLAEKVVYVGVWIPALLLTIPDFIFANVSEADDRYI
*************************************************.**********
AAF89349.1 CDRFYPNDLWVVVFQFQHIMVGLILPGIVILSCYCIIISKLSHSKGHQKRKALKTTVILI
AAF42991.1 CDRFYPNDLWVVVFQFQHIMVGLILPGIVMLSCYCIIISKLSHSKGHQKRKALKTTVILI
*****************************:******************************
AAF89349.1 LAFFACWLPYYIGISIDSFILLEIIKQGCEFENTVHKWISITEALAFFHCCLNPILYAFL
AAF42991.1 LAFFACWLPYYIGISIDSFILLEIIKQGCEFENTVHKWISITEALAFFHCCLNPILYAFL
************************************************************
AAF89349.1 GAKFKTSAQHALTSVSRGSSLKILSKGKRGGHSSVSTESESSSFHSS
AAF42991.1 GAKFKTSAQHALTSVSRGSSLKILSKGKRGGHSSVSTESESS-----
******************************************
They came from different labs:
AF178083_1 --> Department of Biology, California State University, Northridge (provides link to publication)
AF172229_1 --> Lab of Cellular and Molecular Evolution, Kunming Institute of Zoology, The Chinese Academy of Sciences (unpublished data)
Accordingly, my guess is that they probably come from different samples. Just like most human genes have variations, these probably came from different individual gibbons (with slightly different versions of the gene)
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