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5.1 years ago
vaish01kv
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How to calculate Gbases? I found a column exclusively for Gbases of read 1 & read 2 of 6.18 and total Gbase of 18.3 in a report. How do I interpret? How is GBases related to Quality?
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Your question needs to be significantly improved before you can get meaningful answers. Provide context of what report are you referring to? What kind of sequencing this is?
It is not related. Unless you are purely referring to getting much less sequence than what the company specifies in technical specification for a type of sequencer.
Its a Pre-alignment QC report generated using FastQC of Exome sequencing.
FastQC generates a qualitative QC report of sequence data. It is not possible to say anything about QC as it relates to exome at this point. You will need to do the alignments and then start looking at specific QC metrics as they relate to the regions you captured in your data.