Course: Approaches and Computational tools for CRISPR Genome editing
Where: Free University of Berlin
When: 28-30 October 2019
OVERVIEW
Clustered regularly interspaced short palindromic repeats (CRISPR) genome editing has revolutionized the ability to modify a genome of interest in a targeted and programmable way. This system has accelerated biological discovery and clinical treatments and new genome editing variants with different editing properties are being introduced at a rapid pace. A variety of computational tools have been developed to address key challenges in the design and analysis of genome editing experiments. In this course we will introduce common design and analysis strategies, as well as state-of-the-art methods and tools that attendees can use to address their own genome editing needs.
COURSE FORMAT
The course will be delivered over the course of three days. Each day will include a lecture and laboratory component. The lecture will introduce the topics of discussion and the laboratory sessions will be focused on practical hands-on analysis of genome editing data. These sessions will involve a combination of both mirroring exercises with the instructor to demonstrate a skill as well as applying these skills on your own to complete individual exercises. After and during each exercise, interpretation of results will be discussed as a group. Computing will be done using a combination of tools installed on the attendees laptop computer and web resources accessed via web browser.
TARGET AUDIENCE & ASSUMED BACKGROUND
This course is aimed at researchers and technical workers who are designing CRISPR genome editing experiments or will be analyzing genome editing data. The material is suitable both for experimentalists who want to learn more about data analysis as well as computational biologists who want to learn about genome editing design and analysis methods.
The requirements for this course are:
- Working knowledge of CRISPR genome editing techniques, including genome-wide screens and tiling screens.
- Also a working knowledge of basic laboratory processes such as PCR, next-generation sequencing, etc.
- Some experience running simple commands on a terminal (Linux, Windows, or OSX)
Course program: https://www.physalia-courses.org/courses-workshops/course53/curriculum53/
Should you have any questions: info@physalia-courses.org
