Questions from beginner: Barcode of Single cell RNA-seq
Hi there, I am a freshman in this area. When I reading the review of it, I am a little bit confused by the barcode of it. In the review, it said barcode is added by PCR. So it is on the end of the sequence.
My question is:
When we doing the NGS, the sequences will go through 'fragmentation' step. So how can the barcode does its job? I mean, just some of the fragment will carry the barcode (those near the end of sequences). How can other fragments be classified into different cells?
Many thanks.
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This may be useful: http://core-genomics.blogspot.com/2016/12/10x-genomics-updates.html